use the align_yaxis() function:
import numpy as np
import matplotlib.pyplot as plt
def align_yaxis(ax1, v1, ax2, v2):
"""adjust ax2 ylimit so that v2 in ax2 is aligned to v1 in ax1"""
_, y1 = ax1.transData.transform((0, v1))
_, y2 = ax2.transData.transform((0, v2))
inv = ax2.transData.inverted()
_, dy = inv.transform((0, 0)) - inv.transform((0, y1-y2))
miny, maxy = ax2.get_ylim()
ax2.set_ylim(miny+dy, maxy+dy)
fig = plt.figure()
ax1 = fig.add_subplot(111)
ax2 = ax1.twinx()
ax1.bar(range(6), (2, -2, 1, 0, 0, 0))
ax2.plot(range(6), (0, 2, 8, -2, 0, 0))
align_yaxis(ax1, 0, ax2, 0)
plt.show()
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