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asp.net - HTTP Error 401.3 - Unauthorized

I am getting this errror in my newly created website in Windows 7 and IIS 7.5. I created an SSL certificate and done binding new website.

HTTP Error 401.3 - Unauthorized
You do not have permission to view this directory or page because of the access control list (ACL) configuration or encryption settings for this resource on the Web server.

Earlier when I created the website, I selected 'application user(pass through authentication)' in 'Add Website' dialogue. and when I click "Test Settings ..." button, I get this error message:

The server is configured to use pass-through authentication with a built-in account to access the specified physical path. However, IIS Manager cannot verify whether the built-in account has access. Make sure that the application pool identity has Read access to the physical path. If this server is joined to a domain, and the application pool identity is NetworkService or LocalSystem, verify that $ has Read access to the physical path. Then test these settings again.

Please see attached image

Please suggest solution to this.

Thanks.

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I had the same issue, ensured my DefaultAppPool was running under ApplicationPoolIdentity and the DefaultAppPool had permissions to the folder, but some files still didn't load.

Turns out my problem was that the anonymous authentication user was set to someone else instead of ApplicationPoolIdentity

Screenshot enter image description here


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